Human Stem Cell Culture Techniques

By junying yu and james a.

Human stem cell culture techniques.

However the successful culture and maintenance of human pluripotent stem cells hpscs in an undifferentiated state requires additional considerations to ensure that cells maintain their key characteristics of self renewal and. Human mesenchymal stem cells hmscs are undoubtedly one of the most promising types of adult stem cells ascs for cell based therapies currently being tested in clinical trials for a wide range of ailments e g brain and spinal cord injury cardiovascular disease and myocardial infarction mi type i diabetes multiple sclerosis crohn s disease cartilage and bone injury. In vivo when the study involves living biological entities within the organism. Different types of stem cells often require different culture conditions.

Human cell culture is practiced in almost every biomedical laboratory throughout the world. Stem cells are unspecialized undifferentiated cells that are characteristically of the same family type lineage. Culture of human stem cells edited by r. The culture of human pluripotent stem cells shares many of the same protocols as standard mammalian cell culture.

Stem cell culture remains a challenge even with the knowledge that scientists have gained over the past decade. For example human induced pluripotent stem cells ipsc and human embryonic stem cells hesc must be cultured on coated plates with a supporting layer of. Preparation of basement membrane extract bme stem cell qualified ecm gel matrix is a soluble basement membrane extract bme purified from engelbreth holm swarm ehs tumors that has been pre qualified for human es ips cell culture the matrix polymerizes at 20 40 c to form a reconstituted basement membrane. Auerbach is a collection of established techniques presented in one volume that is designed to stand alone as a singular definitive resource for anyone interested in the therapeutic potential of human stem cells.

The major application of human cell culture is in stem cell industry where mesenchymal stem cells can be cultured and cryopreserved for future use. Stem cell definitions one of the human body s master cells with the ability to grow into any one of the body s more than 200 cell types. Cell culture basics techniques and media essentially cell culture involves the distribution of cells in an artificial environment in vitro which is composed of the necessary nutrients ideal temperature gases ph and humidity to allow the cells to grow and proliferate. Human cell culture is a widely used laboratory technique that enables cells the building blocks of life to be cultured studied and manipulated under controlled conditions outside of the body.

Cell culture is a fundamental component of tissue culture and tissue engineering as it establishes the basics of growing and maintaining cells in vitro. Ian freshney glyn n. Stacey and jonathan m.