Human Primary Microglia Cells

Primary neurons like all primary cells are isolated directly from human or animal nervous tissue unlike cell lines primary cells maintain the characteristics of their tissue of origin making them a biologically and physiologically relevant tool for the study of neuroscience.

Human primary microglia cells.

Human microglia hm catalog 1900. Human microglia primary cell culture frozen vial. Comments resting hmc3 cells were strongly positive for the microglia macrophage marker iba1 positive for the endotoxin receptor cd14 but negative for the astrocyte marker gfap. Therefore there is a pressing need to develop a renewable source of human microglia such as from induced pluripotent stem cells ipscs.

We are recently preparing the primary astrocytes and microglia cells. Human ipsc derived microglial precursors monocytes were seeded using microglia maintenance medium into 96 well plates at a density of 100 000 cells cm 2. The hmc3 cell line was established through sv40 dependent immortalization of a human fetal brain derived primary microglia culture. Immortalized cells were controlled passaging side by side with the primary cells.

The human microglia cell line was constructed as the bv2 with a retrovirus that should have not infected mammalian cells. We describe a robust method for the derivation of human microglia from stem cells which are phenotypically and functionally comparable with primary microglia. Yet studying human microglia is challenging because of the rarity and difficulty in acquiring primary cells from human fetal or adult cns tissue. The derivation of microglia from human stem cells provides systems for understanding microglial biology and enables functional studies of disease causing mutations.

Tardieu in 1995 through sv40 dependent immortalization of human microglial cells the detailed methodology for the preparation of primary cultures of human microglial cells was previously published briefly microglial cells were isolated by circular shaking from primary mixed cultures of human. During maturation spherical monocytes adhere strongly to the culture surface displaying increasingly ramified morphology as they differentiate to microglia. Microglia are a major glial component of the central nervous system cns play a critical role as resident immunocompetent and phagocytic cells in the cns and serve as scavenger cells in the event of infection inflammation trauma ischemia and neurodegeneration in the cns. Immortalized human microglia im hm provided by innoprot have been developed by immortalizing primary human microglia with sv40 large t antigen.